We used a total of 108 mitochondrial gene sequences for molecular analyses (55 Cyt b sequences and 53 ND4 sequences). Four samples of muscle tissues from S. Sinocyclocheilus angustiporus, S. angularis, and Sinocyclocheilus longicornus sp. nov. were sequenced for two mitochondrial genes and 100 sequences from 45 species of Sinocyclocheilus were downloaded from GenBank. Following Wen et al. (2022), we selected Carassius auratus, Cyprinus carpio, Garra orientalis, Neolissochilus hexagonolepis, Schizothorax yunnanensis, Barbus barbus, Onychostoma simum, Pethia ticto, Myxocyprinus asiaticus, and Danio rerio as outgroup (Table 2).
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